pE-300 white

Please contact us for a quotation.

CoolLED’s pE-300white offers intense, broad-spectrum LED illumination for fluorescence microscopy that is perfect for hospital and regular research laboratory environments. The system is a mercury-free alternative that is safer, more controllable and precise than traditional bulb illumination options. Spectral coverage is from the UV (DAPI excitation) to the red region (Cy5 excitation).

LED technology is quickly evolving, and since 2017 our new generation has double the irradiance at the sample plane, and for even higher irradiance is available as a Direct Fit model (which also reduces the cost to buy and run thanks to fewer consumables). With ACT Label certification, it’s even an environmentally friendly option for illumination.
Please contact us for a quotation.



The pE-300white is a compact and simple to use LED illuminator for microscopes. It provides a broad spectrum of illumination, covering the excitation bands of common fluorophores such as DAPI, CFP, Aqua, FITC, TRITC, TxRed, Cy5 and many more. Operation is by a manual control pod with instant on/off and irradiance control from 0-100%, with remote control available via USB or TTL trigger.
Direct fit or liquid light guide

There are two pE-300white configuration options: direct fit or liquid light guide. The Direct Fit variant delivers higher irradiance and reduces the cost to buy and run thanks to fewer consumables.

For confocal microscopes, the Liquid Light Guide model offers the ideal replacement to a metal halide light source, minimising photobleaching and phototoxicity when viewing samples before scanning. More information on the available variants can be found under specifications.
Fast multi-channel imaging

An additional benefit of the pE-300white is that fluorophores can be viewed either individually or in combination, without filter cube changes. This makes it ideal for use with multi-band filter sets as the screening process can be simplified when fewer filter cubes are used. Independent control of the three LED wavebands allows control of the level of excitation of each fluorophore independently on a multi-stained sample, potentially removing the need for single-band filter sets altogether.
Energy efficiency for cost-saving sustainability

When it comes to making labs safer and more sustainable, LED microscope lighting is an attractive option when compared to old toxic mercury or metal halide lamps. All three channels of the pE-300white at full power use just 60 Watts, which is even lower than other LED technology which uses 120 to 350 Watts. “Green Grants” are even sometimes available from establishments, providing financial support owing to the reduction in institutional energy usage.
Award winning technology

CoolLED’s pE-300 Series was the 2017 winner of the International Institute for Sustainable Laboratories (I2SL) “Go Beyond Award” for “Excellence in sustainability in laboratory and other high technology facilities”.


Remote control via USB or TTL trigger
Clean bright illumination across the spectrum: excites common fluorophores
Three LEDs
Instant on/off: No shutters required, no warm up or cool down
Stable and repeatable: reliable and consistent results
Precise irradiance control in 1% steps (0-100%): no ND filters required


Specify for existing single and multi-band filter sets: no need to buy new filters
Simple to fit, simple to use: no alignment, a once only adjustment
Wide range of microscope adaptors: fits most microscopes